Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve

Journal article

A. Telfer, Monica R. Young, J. Quinn, K. Perez, Crystal N. Sobel, Jayme E Sones, V. Levesque-Beaudin, R. Derbyshire, J. Fernández-Triana, R. Rougerie, A. Thevanayagam, A. Boskovic, A. Borisenko, A. Cadel, Allison Brown, A. Pagès, A. Castillo, A. Nicolai, Barb Mockford Glenn Mockford, Belén Bukowski, B. Wilson, Brock Trojahn, C. A. Lacroix, C. Brimblecombe, Christoper Hay, Christmas Ho, C. Steinke, Connor P. K. Warne, Cristina Garrido Cortes, D. Engelking, Danielle Wright, Darío A. Lijtmaer, D. Gascoigne, David Hernandez Martich, Derek Morningstar, D. Neumann, D. Steinke, Donna DeBruin Marco DeBruin, D. Dobias, E. Sears, E. Richard, Emily S. Damstra, E. Zakharov, F. Laberge, Gemma E Collins, G. Blagoev, Gerrie Grainge, Graham Ansell, G. Meredith, I. Hogg, Jaclyn T A McKeown, Janet Topan, J. Bracey, Jerry Guenther, Jesse Sills-Gilligan, Joseph Addesi, Joshua Persi, K. Layton, K. D'Souza, Kencho Dorji, Kevin Grundy, Kirsti Nghidinwa, K. Ronnenberg, K. Lee, Linxi Xie, Liuqiong Lu, L. Penev, Mailyn A. González, Margaret E. Rosati, Mari Kekkonen, Maria L Kuzmina, Marianne Iskandar, M. Mutanen, M. Fatahi, Mikko Pentinsaari, M. Bauman, N. Nikolova, N. Ivanova, N. Jones, Nimalka Weerasuriya, N. Monkhouse, Pablo D. Lavinia, Paul Jannetta, P. E. Hanisch, R. McMullin, Rafael Ojeda Flores, R. Mouttet, Reid Vender, Renee N Labbee, R. Forsyth, Robert E. Lauder, R. Dickson, R. Kroft, S. Miller, S. MacDonald, S. Panthi, S. Pedersen, S. Sobek-Swant, S. Naik, T. Lipinskaya, T. Eagalle, T. Decaëns, Thibault Kosuth, Thomas W. A. Braukmann, T. Woodcock, T. Roslin, Tony Zammit, Victoria Campbell, V. Dincă, V. Peneva, P. Hebert, Jeremy R. deWaard
Biodiversity Data Journal, 2015

Semantic Scholar DOI PubMedCentral PubMed

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APA Click to copy
Telfer, A., Young, M. R., Quinn, J., Perez, K., Sobel, C. N., Sones, J. E., … deWaard, J. R. (2015). Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve. Biodiversity Data Journal.

Chicago/Turabian Click to copy
Telfer, A., Monica R. Young, J. Quinn, K. Perez, Crystal N. Sobel, Jayme E Sones, V. Levesque-Beaudin, et al. “Biodiversity Inventories in High Gear: DNA Barcoding Facilitates a Rapid Biotic Survey of a Temperate Nature Reserve.” Biodiversity Data Journal (2015).

MLA Click to copy
Telfer, A., et al. “Biodiversity Inventories in High Gear: DNA Barcoding Facilitates a Rapid Biotic Survey of a Temperate Nature Reserve.” Biodiversity Data Journal, 2015.

BibTeX Click to copy

@article{a2015a,
  title = {Biodiversity inventories in high gear: DNA barcoding facilitates a rapid biotic survey of a temperate nature reserve},
  year = {2015},
  journal = {Biodiversity Data Journal},
  author = {Telfer, A. and Young, Monica R. and Quinn, J. and Perez, K. and Sobel, Crystal N. and Sones, Jayme E and Levesque-Beaudin, V. and Derbyshire, R. and Fernández-Triana, J. and Rougerie, R. and Thevanayagam, A. and Boskovic, A. and Borisenko, A. and Cadel, A. and Brown, Allison and Pagès, A. and Castillo, A. and Nicolai, A. and Mockford, Barb Mockford Glenn and Bukowski, Belén and Wilson, B. and Trojahn, Brock and Lacroix, C. A. and Brimblecombe, C. and Hay, Christoper and Ho, Christmas and Steinke, C. and Warne, Connor P. K. and Cortes, Cristina Garrido and Engelking, D. and Wright, Danielle and Lijtmaer, Darío A. and Gascoigne, D. and Martich, David Hernandez and Morningstar, Derek and Neumann, D. and Steinke, D. and DeBruin, Donna DeBruin Marco and Dobias, D. and Sears, E. and Richard, E. and Damstra, Emily S. and Zakharov, E. and Laberge, F. and Collins, Gemma E and Blagoev, G. and Grainge, Gerrie and Ansell, Graham and Meredith, G. and Hogg, I. and McKeown, Jaclyn T A and Topan, Janet and Bracey, J. and Guenther, Jerry and Sills-Gilligan, Jesse and Addesi, Joseph and Persi, Joshua and Layton, K. and D'Souza, K. and Dorji, Kencho and Grundy, Kevin and Nghidinwa, Kirsti and Ronnenberg, K. and Lee, K. and Xie, Linxi and Lu, Liuqiong and Penev, L. and González, Mailyn A. and Rosati, Margaret E. and Kekkonen, Mari and Kuzmina, Maria L and Iskandar, Marianne and Mutanen, M. and Fatahi, M. and Pentinsaari, Mikko and Bauman, M. and Nikolova, N. and Ivanova, N. and Jones, N. and Weerasuriya, Nimalka and Monkhouse, N. and Lavinia, Pablo D. and Jannetta, Paul and Hanisch, P. E. and McMullin, R. and Flores, Rafael Ojeda and Mouttet, R. and Vender, Reid and Labbee, Renee N and Forsyth, R. and Lauder, Robert E. and Dickson, R. and Kroft, R. and Miller, S. and MacDonald, S. and Panthi, S. and Pedersen, S. and Sobek-Swant, S. and Naik, S. and Lipinskaya, T. and Eagalle, T. and Decaëns, T. and Kosuth, Thibault and Braukmann, Thomas W. A. and Woodcock, T. and Roslin, T. and Zammit, Tony and Campbell, Victoria and Dincă, V. and Peneva, V. and Hebert, P. and deWaard, Jeremy R.}
}

Abstract

Abstract Background Comprehensive biotic surveys, or ‘all taxon biodiversity inventories’ (ATBI), have traditionally been limited in scale or scope due to the complications surrounding specimen sorting and species identification. To circumvent these issues, several ATBI projects have successfully integrated DNA barcoding into their identification procedures and witnessed acceleration in their surveys and subsequent increase in project scope and scale. The Biodiversity Institute of Ontario partnered with the rare Charitable Research Reserve and delegates of the 6th International Barcode of Life Conference to complete its own rapid, barcode-assisted ATBI of an established land trust in Cambridge, Ontario, Canada. New information The existing species inventory for the rare Charitable Research Reserve was rapidly expanded by integrating a DNA barcoding workflow with two surveying strategies – a comprehensive sampling scheme over four months, followed by a one-day bioblitz involving international taxonomic experts. The two surveys resulted in 25,287 and 3,502 specimens barcoded, respectively, as well as 127 human observations. This barcoded material, all vouchered at the Biodiversity Institute of Ontario collection, covers 14 phyla, 29 classes, 117 orders, and 531 families of animals, plants, fungi, and lichens. Overall, the ATBI documented 1,102 new species records for the nature reserve, expanding the existing long-term inventory by 49%. In addition, 2,793 distinct Barcode Index Numbers (BINs) were assigned to genus or higher level taxonomy, and represent additional species that will be added once their taxonomy is resolved. For the 3,502 specimens, the collection, sequence analysis, taxonomic assignment, data release and manuscript submission by 100+ co-authors all occurred in less than one week. This demonstrates the speed at which barcode-assisted inventories can be completed and the utility that barcoding provides in minimizing and guiding valuable taxonomic specialist time. The final product is more than a comprehensive biotic inventory – it is also a rich dataset of fine-scale occurrence and sequence data, all archived and cross-linked in the major biodiversity data repositories. This model of rapid generation and dissemination of essential biodiversity data could be followed to conduct regional assessments of biodiversity status and change, and potentially be employed for evaluating progress towards the Aichi Targets of the Strategic Plan for Biodiversity 2011–2020.